THE EFFECT OF OINTMENT WITH A NEW ACEXAMIC ACID DERIVATIVE ON THE REPARATIVE PROCESSES OF RAT SKIN DURING THERMAL BURN

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The aim of the work is to study the effect of ointment with a new derivative 2-E-6-M-3-GP N-A-6-AG on the reparative processes of rat skin on an experimental thermal burn model.
Material and methods. The object of the study was 45 laboratory nonlinear female rats weighing 140-160 g, which were simulated thermal burn under general anesthesia [1]. From the first day and throughout the experiment, the animals of the experimental group corrected the healing of the burn with applications of 2% ointment with 2-E-6-M-3-GP N-A-6-A. The tissues obtained from the thermal burn zone in all the studied groups on the 7th, 14th, and 21st days of the experiment served as the material for morphological study. Histological preparations were stained with hematoxylin and eosin. The healing rate of the thermal injury was evaluated by the planimetric method at the same time of observation.
Results. During the experiment, it was found that the rate of wound closure was the highest in the rats of the experimental group compared to the control ones. So, on the 7th day of the experiment, the burn area in the animals of the experimental group was 1.2 times less than in the control groups, and on the 14th day it was 3.8 times (45.4 ±3.2 mm2 versus 130.5±4.2 mm2 and 127.8±4.27 mm2, respectively). On the 21st day of the experiment, a connective tissue scar formed at the burn site in the experimental group, whereas in control groups 1 and 2, the damage area was still 46.2±2.15 mm2 and 35.7±2.1 mm2.
During morphological analysis, it was found that on the 7th day of the experiment in the experimental group, the thickness of the scab was 257.3±1.2 microns versus 368.5±19.7 microns and 310.6±5.6 microns in control groups 1 and 2, respectively. The leukocyte shaft was almost not determined, its thickness is 1.3 times less than in rats of control groups 1 and 2. The defect area is filled with granulation tissue of a high degree of maturity, its length is 610.3 ±7.1 microns versus 478.4±34.2 and 456.8±18.6 microns. After 14 days, a thin scab was noted in the experimental group of animals (thickness 172.7±4.12 microns, versus 217.6±14.4 microns and 211.7±7.8 microns). The defect area was filled with granulation tissue consisting of numerous collagen fibers arranged in parallel. On the 21st day in the experimental group, the growth of young epithelium was observed almost over the entire area of the defect with the formation of skin derivatives. Visually, the area of the defect in the control group rats was still clearly visible, whereas in the animals of the experimental group it is practically indistinguishable from intact skin. Epithelialization of the wound defect was completed on average 2.2 days earlier in comparison with the control groups.
Conclusions. The study established the pro-regenerative potential of 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate. This fact is confirmed by the fact that in animals receiving ointment applications, the healing of the burn defect was carried out on average 2.2 days earlier than in the control groups.

About the authors

Marina Anatolievna Petrovskaya

Author for correspondence.
Email: solm1990@mail.ru
ORCID iD: 0000-0003-1193-1778
SPIN-code: 5512-7253
Russian Federation, 4, Sovetskaya str., Tver,170000, Russia

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